DPH5 Antikörper (AA 11-250)
Kurzübersicht für DPH5 Antikörper (AA 11-250) (ABIN7599711)
Target
Alle DPH5 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 11-250
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Verwendungszweck
- Anti-DPH5 Antibody Picoband®
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Kreuzreaktivität (Details)
- No cross-reactivity with other proteins.
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Produktmerkmale
- Anti-DPH5 Antibody Picoband® (ABIN7599711). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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Aufreinigung
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human DPH5 recombinant protein (Position: D11-H250).
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Isotyp
- IgG
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Applikationshinweise
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Carette, J. E., Guimaraes, C. P., Varadarajan, M., Park, A. S., Wuethrich, I., Godarova, A., Kotecki, M., Cochran, B. H., Spooner, E., Ploegh, H. L., Brummelkamp, T. R. Haploid genetic screens in human cells identify host factors used by pathogens. Science 326: 1231-1235, 2009. 2. Liu, S., Milne, G. T., Kuremsky, J. G., Fink, G. R., Leppla, S. H. Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Molec. Cell. Biol. 24: 9487-9497, 2004. 3. Zhang, Q.-H., Ye, M., Wu, X.-Y., Ren, S.-X., Zhao, M., Zhao, C.-J., Fu, G., Shen, Y., Fan, H.-Y., Lu, G., Zhong, M., Xu, X.-R., and 9 others. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res. 10: 1546-1560, 2000. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Konzentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- DPH5 (DPH5 Homolog (DPH5))
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Andere Bezeichnung
- DPH5
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Hintergrund
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Synonyms: Solute carrier family 2, facilitated glucose transporter member 6, Glucose transporter type 6, GLUT-6, Glucose transporter type 9, GLUT-9, SLC2A6, GLUT9
Tissue Specificity: Highly expressed in brain, spleen and peripheral blood leukocytes.
Background: Diphthine synthase is an enzyme that in humans is encoded by the DPH5 gene. This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.
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Molekulargewicht
- 37 kDa
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Gen-ID
- 51611
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UniProt
- Q9H2P9
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Pathways
- SARS-CoV-2 Protein Interaktom
Target
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