NFS1 Antikörper (AA 107-356)

Produktnummer ABIN7599676

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NFS1 in WB, ELISA, IP und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für NFS1 Antikörper (AA 107-356) (ABIN7599676)

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-NFS1 Antikörper

Bindungsspezifität: AA 107-356

Verwendungszweck: Anti-NFS1 Antibody Picoband®

Produktmerkmale: Anti-NFS1 Antibody Picoband® (ABIN7599676). Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NFS1 recombinant protein (Position: R107-L356). Human NFS1 shares 96.4% and 96% amino acid (aa) sequence identity with mouse and rat NFS1, respectively.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alvarez, S. W., Sviderskiy, V. O., Terzi, E. M., Papagiannakopoulos, T., Moreira, A. L., Adams, S., Sabatini, D. M., Birsoy, K., Possemato, R. NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis. Nature 551: 639-643, 2017. Note: Erratum: Nature 609: E12, 2022. 2. Biederbick, A., Stehling, O., Rosser, R., Niggemeyer, B., Nakai, Y., Elsasser, H.-P., Lill, R. Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation. Molec. Cell. Biol. 26: 5675-5687, 2006. 3. Farhan, S. M. K., Wang, J., Robinson, J. F., Lahiry P., Siu, V. M., Prasad, C., Kronick, J. B., Ramsay, D. A., Rupar C. A., Hegele, R. A. Exome sequencing identifies NFS1 deficiency in a novel Fe-S cluster disease, infantile mitochondrial complex II/III deficiency. Molec. Genet. Genomic Med. 2: 73-80, 2014.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NFS1

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Andere Bezeichnung: NFS1

Hintergrund: Cysteine desulfurase, mitochondrial is an enzyme that in humans is encoded by the NFS1 gene. Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH . The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.

Molekulargewicht: 45 kDa

Gen-ID: 9054

UniProt: Q9Y697

Pathways: Transition Metal Ion Homeostasis