ACAT1 Antikörper (AA 10-404)

Produktnummer ABIN7599600

Dieser Anti-ACAT1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von ACAT1 in WB, IHC, ELISA, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ACAT1 Antikörper (AA 10-404) (ABIN7599600)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-ACAT1 Antikörper

Bindungsspezifität: AA 10-404

Verwendungszweck: Anti-ACAT1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ACAT1 Antibody Picoband® (ABIN7599600). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ACAT1 recombinant protein (Position: S10-Q404).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Daum, R. S., Scriver, C. R., Mamer, O. A., Delvin, E., Lamm, P. H., Goldman, H. An inherited disorder of isoleucine catabolism causing accumulation of alpha-methylacetoacetate and alpha-methyl-beta-hydroxybutyrate and intermittent metabolic acidosis. Pediat. Res. 7: 149-160, 1973. 2. Fukao, T., Matsuo, N., Zhang, G. X., Urasawa, R., Kubo, T., Kohno, Y., Kondo, N. Single base substitutions at the initiator codon in the mitochondrial acetoacetyl-CoA thiolase (ACAT1/T2) gene result in production of varying amounts of wild-type T2 polypeptide. Hum. Mutat. 21: 587-592, 2003. 3. Fukao, T., Nakamura, H., Nakamura, K., Perez-Cerda, C., Baldellou, A., Barrionuevo, C. R., Castello, F. G., Kohno, Y., Ugarte, M., Kondo, N. Characterization of six mutations in five Spanish patients with mitochondrial acetoacetyl-CoA thiolase deficiency: effects of amino acid substitutions on tertiary structure. Molec. Genet. Metab. 75: 235-243, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Andere Bezeichnung: ACAT1

Hintergrund:

Synonyms: Bifunctional epoxide hydrolase 2,Cytosolic epoxide hydrolase 2,CEH,3.3.2.10,Epoxide hydratase,Soluble epoxide hydrolase,SEH,Lipid-phosphate phosphatase,3.1.3.76,EPHX2,

Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.

Background: Acetyl-CoA acetyltransferase, mitochondrial, also known as acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT1 (Acetyl-Coenzyme A acetyltransferase 1) gene. This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Molekulargewicht: 40 kDa

Gen-ID: 38

UniProt: P24752