PICALM Antikörper (AA 1-652)

Produktnummer ABIN7599516

Dieses Anti-PICALM-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PICALM in WB, ELISA, IHC, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für PICALM Antikörper (AA 1-652) (ABIN7599516)

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PICALM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-PICALM Antikörper

Bindungsspezifität: AA 1-652

Verwendungszweck: Anti-PICALM Picoband® Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PICALM Picoband® Antibody (ABIN7599516). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PICALM recombinant protein (Position: M1-M652).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Morris, S. A., Schroder, S., Plessmann, U., Weber, K., Ungewickell, E. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site. EMBO J. 12: 667-675, 1993. 2. Stern, J. A., White, S. N., Lehmkuhl, L. B., Reina-Doreste, Y., Ferguson, J. L., Nascone-Yoder, N. M., Meurs, K. M. A single codon insertion in PICALM is associated with development of familial subvalvular aortic stenosis in Newfoundland dogs. Hum. Genet. 133: 1139-1148, 2014. 3. Treusch, S., Hamamichi, S., Goodman, J. L., Matlack, K. E. S., Chung, C. Y., Baru, V., Shulman, J. M., Parrado, A., Bevis, B. J., Valastyan, J. S., Han, H., Lindhagen-Persson, M., and 9 others. Functional links between amyloid-beta toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast. Science 334: 1241-1245, 2011.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PICALM

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Andere Bezeichnung: PICALM

Hintergrund:

Synonyms: Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein, PICALM, CALM

Tissue Specificity: In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78 % of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.

Background: Phosphatidylinositol binding clathrin assembly protein, also known as PICALM, is a protein which in humans is encoded by the PICALM gene. It is mapped to 11q14.2. This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t (10,11) (p13,q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 71 kDa

Gen-ID: 8301

UniProt: Q13492

Pathways: Synaptic Membrane