TRMT2B Antikörper (AA 1-504)

Produktnummer ABIN7599456

Produktdetails anti-TRMT2B Antikörper

Target: TRMT2B (tRNA Methyltransferase 2 Homolog B (TRMT2B))

Bindungsspezifität: AA 1-504

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRMT2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: Anti-TRMT2B Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-TRMT2B Antibody Picoband® (ABIN7599456). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human TRMT2B recombinant protein (Position: M1-R504).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Minczuk, M. , & Powell, C. A. . Trmt2b is responsible for both trna and rrna m5u-methylation in human mitochondria. 2. Dai, W. , Li, A. , Yu, N. J. , Nguyen, T. , Leach, R. W. , & M Wühr, et al. Activity-based rna-modifying enzyme probing reveals dus3l-mediated dihydrouridylation. Nature chemical biology. 3. Daakour, S. , Hajingabo, L. J. , Kerselidou, D. , Devresse, A. , Kettmann, R. , & Simonis, N. , et al. (2016). Systematic interactome mapping of acute lymphoblastic leukemia cancer gene products reveals ext-1 tumor suppressor as a notch1 and fbwx7 common interactor. BMC Cancer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu TRMT2B

Target: TRMT2B (tRNA Methyltransferase 2 Homolog B (TRMT2B))

Andere Bezeichnung: TRMT2B (TRMT2B Produkte)

Hintergrund:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms.

Molekulargewicht: 47 kDa

Gen-ID: 79979