FLI1 Antikörper (AA 1-452)

Produktnummer ABIN7599409

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch FLI1 in WB, ELISA, IHC, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für FLI1 Antikörper (AA 1-452) (ABIN7599409)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FLI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-FLI1 Antikörper

Bindungsspezifität: AA 1-452

Verwendungszweck: Anti-FLI1 Picoband® Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-FLI1 Picoband® Antibody (ABIN7599409). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human FLI1 recombinant protein (Position: M1-Y452).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Lin, P. P., Brody, R. I., Hamelin, A. C., Bradner, J. E., Healey, J. H., Ladanyi, M. Differential transactivation by alternative EWS-FLI1 fusion proteins correlates with clinical heterogeneity in Ewing's sarcoma. Cancer Res. 59: 1428-1432, 1999. 2. May, W. A., Gishizky, M. L., Lessnick, S. L., Lunsford, L. B., Lewis, B. C., Delattre, O., Zucman, J., Thomas, G., Denny, C. T. Ewing sarcoma 11,22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation. Proc. Nat. Acad. Sci. 90: 5752-5756, 1993. 3. Nakatani, F., Tanaka, K., Sakimura, R., Matsumoto, Y., Matsunobu, T., Li, X., Hanada, M., Okada, T., Iwamoto, Y. Identification of p21(WAF1/CIP1) as a target of EWS-Fli1 oncogenic fusion protein. J. Biol. Chem. 278: 15105-15115, 2003.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Andere Bezeichnung: FLI1

Hintergrund:

Synonyms: Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1

Tissue Specificity: Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.

Background: Friend leukemia integration 1 transcription factor (FLI1), also known as transcription factor ERGB, is a protein that in humans is encoded by the FLI1 gene, which is a proto-oncogene. It is mapped to 11q24.3. This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t (11,22) (q24,q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4,11) (q21,q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 54 kDa

Gen-ID: 2313

UniProt: Q01543