PPARA Antikörper (AA 1-426)

Produktnummer ABIN7599384

Dieses Anti-PPARA-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PPARA in WB, ELISA und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für PPARA Antikörper (AA 1-426) (ABIN7599384)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPARA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PPARA Antikörper

Bindungsspezifität: AA 1-426

Verwendungszweck: Anti-PPAR alpha/PPARA Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PPAR alpha/PPARA Antibody Picoband® (ABIN7599384). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PPAR alpha/PPARA recombinant protein (Position: M1-L426).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Sher T, Yi HF, McBride OW, Gonzalez FJ (June 1993). "cDNA cloning, chromosomal mapping, and functional characterization of the human peroxisome proliferator activated receptor". Biochemistry 32 (21): 5598-604. doi:10.1021/bi00072a015. PMID 7684926. 2. Kersten S, Seydoux J, Peters JM, Gonzalez FJ, Desvergne B, Wahli W. (June 1999). "Peroxisome proliferator-activated receptor alpha mediates the adaptive response to fasting.". J Clin Invest. 103 (11): 1489-98. doi:10.1172/JCI6223. PMC 408372. PMID 10359558. 3. Jung, D., Fried, M., Kullak-Ublick, G. A.Human apical sodium-dependent bile salt transporter gene (SLC10A2) is regulated by the peroxisome proliferator-activated receptor alpha.J. Biol. Chem.277: 30559-30566, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Andere Bezeichnung: PPARA

Hintergrund:

Synonyms: Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein, GJA1, GJAL

Tissue Specificity: Expressed in the heart and fetal cochlea.

Background: Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85 % and 91 % DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

Molekulargewicht: 52 kDa

Gen-ID: 5465

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C