RAD18 Antikörper (AA 1-408)

Produktnummer ABIN7599364

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RAD18 in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für RAD18 Antikörper (AA 1-408) (ABIN7599364)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-RAD18 Antikörper

Bindungsspezifität: AA 1-408

Verwendungszweck: Anti-RAD18 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-RAD18 Antibody Picoband® (ABIN7599364). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human RAD18 recombinant protein (Position: M1-D408).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Adams, D. J., van der Weyden, L., Gergely, F. V., Arends, M. J., Ng, B. L., Tannahill, D., Kanaar, R., Markus, A., Morris, B. J., Bradley, A. BRCTx is a novel, highly conserved RAD18-interacting protein. Molec. Cell. Biol. 25: 779-788, 2005. 2. Branzei, D., Vanoli, F., Foiani, M. SUMOylation regulates Rad18-mediated template switch. Nature 456: 915-920, 2008. 3. Centore, R. C., Yazinski, S. A., Tse, A., Zou, L. Spartan/C1orf124, a reader of PCNA ubiquitylation and a regulator of UV-induced DNA damage response. Molec. Cell 46: 625-635, 2012.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Andere Bezeichnung: RAD18

Hintergrund:

Synonyms: Guanine deaminase, Guanase, Guanine aminase, Guanine aminohydrolase, GAH, p51-nedasin, GDA, KIAA1258

Tissue Specificity: Expressed in lung carcinoma cell lines but not in normal lung tissues.

Background: E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Molekulargewicht: 75 kDa

Gen-ID: 56852