LYAR Antikörper (AA 1-379)

Produktnummer ABIN7599330

Dieses Anti-LYAR-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LYAR in WB, ELISA, ICC und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für LYAR Antikörper (AA 1-379) (ABIN7599330)

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LYAR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-LYAR Antikörper

Bindungsspezifität: AA 1-379

Verwendungszweck: Anti-LYAR Antibody Picoband®

Produktmerkmale: Anti-LYAR Antibody Picoband® (ABIN7599330). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human LYAR recombinant protein (Position: M1-K379). Human LYAR shares 71.9% and 73.7% amino acid (aa) sequence identity with mouse and rat LYAR, respectively.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 9/21/2017. 2. Ju, J., Wang, Y., Liu, R., Zhang, Y., Xu, Z., Wang, Y., Wu, Y., Liu, M., Cerruti, L., Zou, F., Ma, C., Fang, M., Tan, R., Jane, S. M., Zhao, Q. Human fetal globin gene expression is regulated by LYAR. Nucleic Acids Res. 42: 9740-9752, 2014. 3. Su, L., Hershberger, R. J., Weissman, I. L. LYAR, a novel nucleolar protein with zinc finger DNA-binding motifs, is involved in cell growth regulation. Genes Dev. 7: 735-748, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu LYAR

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Andere Bezeichnung: LYAR

Substanzklasse: Antibody

Hintergrund: Cell growth-regulating nucleolar protein is a protein that in humans is encoded by the LYAR gene (Ly-1 antibody reactive clone). LYAR (Ly-1/CD5 antibody reactive clone) is a 45-50 kDa nucleolar protein that was named for the ability of its antibody to cross-react with Ly-1. Its function is unclear, it is known to associate with MYCN and RRP1B, the latter association giving rise to the suggestion that LYAR is involved with RNA metabolism. Human LYAR is 379 amino acids (aa) in length. It contains two C2H2-type Zn finger regions (aa 6-25 and 33-51) followed by one coiled-coil region (aa 175-219) and an NLS (aa 217-222). There are multiple Zn-binding sites and three utilized phosphorylation sites at Ser244, Ser258 and Ser276. Over aa 288-379, human LYAR shares 75 % aa identity with mouse LYAR.

Molekulargewicht: 44 kDa

Gen-ID: 55646