PPIE Antikörper (AA 1-301)

Produktnummer ABIN7599242

Der Maus Monoklonal Anti-PPIE Antikörper wurde für WB, IHC, ICC, IF und FACS validiert. Er ist geeignet, PPIE in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PPIE Antikörper (AA 1-301) (ABIN7599242)

Target: PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PPIE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 4I9

Produktdetails anti-PPIE Antikörper

Bindungsspezifität: AA 1-301

Verwendungszweck: Anti-Cyclophilin E/PPIE Antibody Picoband® (monoclonal, 4I9)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Cyclophilin E/PPIE Antibody Picoband® (monoclonal, 4I9) (ABIN7599242). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclophilin E/PPIE recombinant protein (Position: M1-V301).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Kim, J.-O., Nau, M. M., Allikian, K. A., Makela, T. P., Alitalo, K., Johnson, B. E., Kelley, M. J. Co-amplification of a novel cyclophilin-like gene (PPIE) with L-myc in small cell lung cancer cell lines. Oncogene 17: 1019-1026, 1998. 2. Mi, H., Kops, O., Zimmermann, E., Jaschke, A., Tropschug, M. A nuclear RNA-binding cyclophilin in human T cells. FEBS Lett. 398: 201-205, 1996.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PPIE

Target: PPIE (Peptidylprolyl Isomerase E (Cyclophilin E) (PPIE))

Andere Bezeichnung: PPIE

Hintergrund:

Synonyms: T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

Molekulargewicht: 35 kDa

Gen-ID: 10450