NUF2 Antikörper (AA 1-297)

Produktnummer ABIN7599236

Der Kaninchen Polyklonal Anti-NUF2-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, NUF2 in Proben von Human zu detektieren.

Kurzübersicht für NUF2 Antikörper (AA 1-297) (ABIN7599236)

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-NUF2 Antikörper

Bindungsspezifität: AA 1-297

Verwendungszweck: Anti-NUF2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NUF2 Antibody Picoband® (ABIN7599236). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NUF2 recombinant protein (Position: M1-Q297).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. DeLuca, J. G., Moree, B., Hickey, J. M., Kilmartin, J. V., Salmon, E. D. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J. Cell Biol. 159: 549-555, 2002. 2. Liu, D., Ding, X., Du, J., Cai, X., Huang, Y., Ward, T., Shaw, A., Yang, Y., Hu, R., Jin, C., Yao, X. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J. Biol. Chem. 282: 21415-21424, 2007. 3. Nabetani, A., Koujin, T., Tsutsumi, C., Haraguchi, T., Hiraoka, Y. A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. Chromosoma 110: 322-334, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NUF2

Target: NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))

Andere Bezeichnung: NUF2

Hintergrund:

Synonyms: S-arrestin, 48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin, SAG,

Tissue Specificity: Retina and pineal gland.

Background: Kinetochore protein Nuf2 is a protein that in humans is encoded by the NUF2 gene. This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.

Molekulargewicht: 54 kDa

Gen-ID: 83540

Pathways: Maintenance of Protein Location