NDUFV2 Antikörper (AA 1-249)

Produktnummer ABIN7599154

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NDUFV2 in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für NDUFV2 Antikörper (AA 1-249) (ABIN7599154)

Target: NDUFV2 (NADH Dehydrogenase (Ubiquinone) Flavoprotein 2, 24kDa (NDUFV2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NDUFV2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-NDUFV2 Antikörper

Bindungsspezifität: AA 1-249

Verwendungszweck: Anti-NDUFV2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NDUFV2 Antibody Picoband® (ABIN7599154). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NDUFV2 recombinant protein (Position: M1-L249).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Benit, P., Beugnot, R., Chretien, D., Giurgea, I., De Lonlay-Debeney, P., Issartel, J.-P., Corral-Debrinski, M., Kerscher, S., Rustin, P., Rotig, A., Munnich, A. Mutant NDUFV2 subunit of mitochondrial complex I causes early onset hypertrophic cardiomyopathy and encephalopathy. Hum. Mutat. 21: 582-586, 2003. 2. Cameron, J. M., MacKay, N., Feigenbaum, A., Tarnopolsky, M., Blaser, S., Robinson, B. H., Schulze, A. Exome sequencing identifies complex I NDUFV2 mutations as a novel cause of Leigh syndrome. Europ. J. Paediat. Neurol. 19: 525-532, 2015. 3. de Coo, R., Buddiger, P., Smeets, H., Geurts van Kessel, A., Morgan-Hughes, J., Weghuis, D. O., Overhauser, J., van Oost, B. Molecular cloning and characterization of the active human mitochondrial NADH:ubiquinone oxidoreductase 24- kDa gene (NDUFV2) and its pseudogene. Genomics 26: 461-466, 1995.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NDUFV2

Target: NDUFV2 (NADH Dehydrogenase (Ubiquinone) Flavoprotein 2, 24kDa (NDUFV2))

Andere Bezeichnung: NDUFV2

Hintergrund:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 16, Deubiquitinating enzyme 16, Ubiquitin thioesterase 16, Ubiquitin-processing protease UBP-M, Ubiquitin-specific-processing protease 16, USP16, MSTP039

Tissue Specificity: Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (NDUFV2) is an enzyme that in humans is encoded by the NDUFV2 gene. The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19.

Molekulargewicht: 27 kDa

Gen-ID: 4729

UniProt: P19404