C21ORF45 Antikörper (AA 1-233)

Produktnummer ABIN7599127

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch C21ORF45 in WB, ELISA, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für C21ORF45 Antikörper (AA 1-233) (ABIN7599127)

Target: C21ORF45 (MIS18A) (MIS18 Kinetochore Protein Homolog A (MIS18A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser C21ORF45 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-C21ORF45 Antikörper

Bindungsspezifität: AA 1-233

Verwendungszweck: Anti-C21orf45/MIS18A Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-C21orf45/MIS18A Antibody Picoband® (ABIN7599127). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human C21orf45/MIS18A recombinant protein (Position: M1-D233).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fujita, Y., Hayashi, T., Kiyomitsu, T., Toyoda, Y., Kokubu, A., Obuse, C., Yanagida, M. Priming of centromere for CENP-A recruitment by human hMi18-alpha, hMis18-beta, and M18BP1. Dev. Cell 12: 17-30, 2007. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/28/2018. 3. Kim, I. S., Lee, M., Park, K. C., Jeon, Y., Park, J. H., Hwang, E. J., Jeon, T. I., Ko, S., Lee, H., Baek, S. H., Kim, K. I. Roles of Mis18-alpha in epigenetic regulation of centromeric chromatin and CENP-A loading. Molec. Cell 46: 260-273, 2012.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu C21ORF45

Target: C21ORF45 (MIS18A) (MIS18 Kinetochore Protein Homolog A (MIS18A))

Andere Bezeichnung: MIS18A

Hintergrund:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: Protein Mis18-alpha is a protein that in humans is encoded by the MIS18A gene. C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer (~52 kDa) and heterodimer(~105 kDa) forms.

Molekulargewicht: 26 kDa

Gen-ID: 54069