RIOK1 Antikörper (AA 1-222)

Produktnummer ABIN7599108

Dieses Anti-RIOK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RIOK1 in WB, ELISA und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für RIOK1 Antikörper (AA 1-222) (ABIN7599108)

Target: RIOK1 (RIO Kinase 1 (RIOK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RIOK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-RIOK1 Antikörper

Bindungsspezifität: AA 1-222

Verwendungszweck: Anti-RIOK1/RIO1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-RIOK1/RIO1 Antibody Picoband® (ABIN7599108). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human RIOK1/RIO1 recombinant protein (Position: M1-K222).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ameismeier, M., Cheng, J., Berninghausen, O., Beckmann, R. Visualizing late states of human 40S ribosomal subunit maturation. Nature 558: 249-253, 2018. 2. Guderian, G., Peter, C., Wiesner, J., Sickmann, A., Schulze-Osthoff, K., Fischer, U., Grimmler, M. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. J. Biol. Chem. 286: 1976-1986, 2011 3. Hartz, P. A. Personal Communication. Baltimore, Md. 10/31/2017.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu RIOK1

Target: RIOK1 (RIO Kinase 1 (RIOK1))

Andere Bezeichnung: RIOK1

Hintergrund:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: Serine/threonine-protein kinase RIO1 is an enzyme that in humans is encode by the RIOK1 gene. The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits.

Molekulargewicht: 66 kDa

Gen-ID: 83732