MRPS25 Antikörper (AA 1-173)

Produktnummer ABIN7599032

Der Kaninchen Polyklonal Anti-MRPS25-Antikörper wurde für WB, ELISA, FACS, IHC, IF und ICC validiert. Er ist geeignet, MRPS25 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MRPS25 Antikörper (AA 1-173) (ABIN7599032)

Target: MRPS25 (Mitochondrial Ribosomal Protein S25 (MRPS25))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MRPS25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-MRPS25 Antikörper

Bindungsspezifität: AA 1-173

Verwendungszweck: Anti-MRPS25 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-MRPS25 Antibody Picoband® (ABIN7599032). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MRPS25 recombinant protein (Position: M1-D173).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bugiardini, E., Mitchell, A. L., Dalla Rosa, I., Horning-Do, H.-T., Pitmann, A. M., Poole, O. V., Holton, J. L., Shah, S., Woodward, C., Hargreaves, I., Quinlivan, R., Amunts, A., Wiesner, R. J., Houlden, H., Holt, I. J., Hanna, M. G., Pitceathly, R. D. S., Spinazzola, A. MRPS25 mutations impair mitochondrial translation and cause encephalomyopathy. Hum. Molec. Genet. 28: 2711-2719, 2019. 2. Kenmochi, N., Suzuki, T., Uechi, T., Magoori, M., Kuniba, M., Higa, S., Watanabe, K., Tanaka, T. The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. Genomics 77: 65-70, 2001. 3. Koc, E. C., Burkhart, W., Blackburn, K., Moseley, A., Spremulli, L. L. The small subunit of the mammalian mitochondrial ribosome: identification of the full complement of ribosomal proteins present. J. Biol. Chem. 276: 19363-19374, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu MRPS25

Target: MRPS25 (Mitochondrial Ribosomal Protein S25 (MRPS25))

Andere Bezeichnung: MRPS25

Hintergrund:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: 28S ribosomal protein S25, mitochondrial is a protein that in humans is encoded by the MRPS25 gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 20 kDa

Gen-ID: 64432

UniProt: P82663

Pathways: SARS-CoV-2 Protein Interaktom