C3b/iC3b Antikörper (PE)

Produktnummer ABIN7595388

Der Maus Monoklonal anti-C3b/iC3b Antikörper wird verwendet zum Nachweis von C3b/iC3b in Proben von Human und Non-Human Primate. Er wurde validiert für FACS.

Kurzübersicht für C3b/iC3b Antikörper (PE) (ABIN7595388)

Target: C3b/iC3b

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS)

Klon: 3E7

Produktdetails

Verwendungszweck: Anti-Hu C3b/iC3b PE

Produktmerkmale: The mouse monoclonal antibody 3E7 recognizes complement component C3b and iC3b. It does not cross-react with C3d.

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: human complement component C3b

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze,Protect from prolonged exposure to light

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Antigendetails

Target: C3b/iC3b

Hintergrund:

NCBI Full Gene Name: complement C3

Description: Complement component C3 plays a key role in the activation of complement system. In classical complement system pathway the activated C2 and C4 form classical C3-convertase (C4b2b) which cleaves C3 into C3a and C3b. In alternative complement system pathway C3 is cleaved by alternative C3-convertase (C3bBb), composed of C3b (which can be generated also by spontaneous hydrolysis of C3) and the activated form of factor B. C3b activates downstream cascade leading to formation of pores in the plasma membrane of attacked cell. C3b and its proteolytically inactive form iC3b also serve as important opsonins. C3b can generate C3f, as well as the iC3b can be further cleaved into C3c, C3d and C3g. Complement system is regulated by effective inactivation of free C3b by factor H and factor I. C3b attached to the surface of its target is protected from this inactivation. Complement system is also regulated by other proteins, e.g. CD35, CD46, CD55, or CD59. Undesired activation of complement cascade can lead to severe diseases such as PNH, rheumatoid arthritis, macular degeneration and other. Recently it has been demonstrated to take part in complications associated with Covid-19.

Other names: complement component C3, complement component C3b, complement component iC3b

Gene name: C3

Gen-ID: 718

UniProt: P01024