NrCAM Antikörper (Extracellular) (FITC)

Produktnummer ABIN7582053

Dieses Anti-NrCAM-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NrCAM in FACS und LCI. Geeignet für Ratte.

Kurzübersicht für NrCAM Antikörper (Extracellular) (FITC) (ABIN7582053)

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NrCAM Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-NrCAM Antikörper (FITC)

Bindungsspezifität: AA 583-597, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC

Sequenz: (C)KDNGELPN DERFSVD

Spezifität: Extracellular, N-terminus

Homologie: human - 13,16 amino acid residues identical,Mouse - 15

Produktmerkmale: Anti-NrCAM (extracellular) Antibody (ABIN7043369, ABIN7044690 and ABIN7044691) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NrCAM from mouse, rat and human samples. Anti-NrCAM (extracellular)-FITC Antibody (ABIN7043369, ABIN7044690 and ABIN7044691-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NrCAM from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236074)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Andere Bezeichnung: NRCAM

Hintergrund: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo,NrCAM (Neuron-glia related cell adhesion molecule) is a cell adhesion molecule that belongs to the immunoglobulin-class (Ig) of the L1 family. NrCAM promotes axon growth and repulsion through trans homophilic binding of extracellular domains and intracellular coupling of the conserved cytoplasmic domains to the actin cytoskeleton. This interaction occurs through ankyrin B and ezrin-radixin-moesin adaptors. In addition, NrCAM is responsible for controlling dendritic spine densities, axonal guidance and targeting, and neurite outgrowth by acting as a co-receptor molecule at the neuronal cell surface1,2.NrCAM is highly expressed in the cortex1,3. Its structure contains a PDZ binding motif with the potential to bind PSD scaffold proteins PSD-95 and SAP102. The Ig domains of the protein mediate homophilic and heterophilic binding to multiple ligands2,3.Genetic polymorphisms in the NrCAM gene are linked to autism spectrum disorders, intellectual disability and addictive behavior. NrCAM knockout mice demonstrate autism spectrum disorder related behaviors including impaired sociability, cognitive inflexibility, hypersensitivity to sensory stimuli and altered visual cortical responses.

Gen-ID: 497815

UniProt: P97686

Pathways: Regulation of Cell Size