HRH1 Antikörper (Extracellular) (FITC)

Produktnummer ABIN7582039

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HRH1 in FACS. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für HRH1 Antikörper (Extracellular) (FITC) (ABIN7582039)

Target: HRH1 (Histamine Receptor H1 (HRH1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HRH1 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Produktdetails anti-HRH1 Antikörper (FITC)

Bindungsspezifität: AA 176-191, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC

Sequenz: (C)REDK(S)ETDFYNVTWF K

Spezifität: 2nd extracellular loop.

Homologie: Mouse, human - 14,16 amino acid residues identical,rat - 15

Produktmerkmale: Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (ABIN7043269, ABIN7044512 and ABIN7044513) is a highly specific antibody directed against an extracellular epitope of the mouse H1R. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize H1R  from mouse, rat and human samples. Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody (ABIN7043269, ABIN7044512 and ABIN7044513-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize H1R from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235747)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Andere Bezeichnung: HRH1

Hintergrund: Histamine Receptor H1, H1R,Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.H1 receptor is excitatory and couples to Gq-type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.

Gen-ID: 15465

UniProt: P70174

Pathways: Regulation of Carbohydrate Metabolic Process