GPR4 Antikörper (Extracellular) (FITC)

Produktnummer ABIN7582035

Dieses Anti-GPR4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GPR4 in FACS und LCI. Geeignet für Ratte.

Kurzübersicht für GPR4 Antikörper (Extracellular) (FITC) (ABIN7582035)

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR4 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR4 Antikörper (FITC)

Bindungsspezifität: AA 162-177, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR4 (extracellular) conjugated to the fluorescent dye FITC

Sequenz: (C)RDRYNHTF(S)FEKFPME

Spezifität: 2nd extracellular loop

Homologie: Rat,mouse,human - 15,16 amino acid residues identical

Produktmerkmale: Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize GPR4 from mouse, rat and human samples. Anti-GPR4 (extracellular)-FITC Antibody (ABIN7043160, ABIN7044423 and ABIN7044424-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR4 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235415)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR4

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Andere Bezeichnung: GPR4

Hintergrund: Acid-sensing G-protein coupled receptor 4, GPR19,G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

Gen-ID: 308408

UniProt: Q4KLH9