GPR142 Antikörper (Extracellular) (FITC)

Produktnummer ABIN7582032

Dieses Anti-GPR142-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GPR142 in FACS und LCI. Geeignet für Maus.

Kurzübersicht für GPR142 Antikörper (Extracellular) (FITC) (ABIN7582032)

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR142 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR142 Antikörper (FITC)

Bindungsspezifität: AA 291-304, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR142 (extracellular) conjugated to the fluorescent dye FITC

Sequenz: (C)APVHRDWRVH LALD

Spezifität: Extracellular, 3rd loop.

Homologie: Rat,human - identical

Produktmerkmale: Anti-GPR142 (extracellular) Antibody (ABIN7237652, ABIN7316613 and ABIN7316614) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR142 from rat, mouse and human samples. Anti-GPR142 (extracellular)-FITC Antibody (ABIN7237652, ABIN7316613 and ABIN7316614-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235372)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR142

Target: GPR142 (G Protein-Coupled Receptor 142 (GPR142))

Andere Bezeichnung: GPR142

Hintergrund: G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2,G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33 % amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.

Gen-ID: 217302

UniProt: Q7TQN9