Tetraspanin 2 Antikörper (Extracellular, Loop 2)

Produktnummer ABIN7886697

Der Kaninchen Polyklonal anti-Tetraspanin 2 Antikörper wird verwendet zum Nachweis von Tetraspanin 2 in Proben von Maus. Er wurde validiert für WB, FACS, IHC und LCI.

Kurzübersicht für Tetraspanin 2 Antikörper (Extracellular, Loop 2) (ABIN7886697)

Target: Tetraspanin 2 (TSPAN2)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tetraspanin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Produktdetails anti-Tetraspanin 2 Antikörper

Bindungsspezifität: AA 114-130, Extracellular, Loop 2

Verwendungszweck: A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)

Sequenz: (C)DVAIRHVQSM YEEAYSD

Homologie: Rat - identical,Human - 14 out of 17 amino acid residues identical

Produktmerkmale: Anti-Tetraspanin 2 (extracellular) Antibody (ABIN7581998) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize TSPAN2 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DVAIRHVQSMYEEAYSD, corresponding to amino acid residues 114 - 130 of mouse TSPAN2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Tetraspanin 2

Target: Tetraspanin 2 (TSPAN2)

Andere Bezeichnung: TSPAN2

Hintergrund:

Synonyms: Tetraspan NET-3, TSPAN2

Description: Tetraspanin 2 (TSPAN2) is a member of the Tetraspanin family of proteins, that comprises 33 members in humans. The family is characterized by the presence of four transmembrane domains, which give rise to the name "tetraspanin", as well as the presence two extracellular loops (EC1 and EC2), a small cytoplasmic loop, and short N- and C-terminal tails 1,2.Tetraspanins are able to interact with each other and also with numerous partner proteins such as integrins, growth factor receptors and ion channels, to create a dynamic network of interactions known as the tetraspanin-enriched microdomains (TEMs). Consequently, tetraspanins are regarded as molecular organizers and by forming complexes with these proteins, tetraspanins can modulate signal transduction pathways and cellular responses 2,3,4.Although TSPAN2 shares a relatively high homology with well-studied tetraspanins like CD9 (TSPAN29) or CD81 (TSPAN28), it has been less well-characterised up until now 3.Nevertheless, TSPAN2, like the related CD9 and CD81 proteins, has been described in relation to exosome formation, specifically regarding neuroinflammation and microglia activation 5, as well as oligodendrocyte differentiation into myelin-forming glia and in the progression of tumor metastasis by modulating cancer cell motility and invasion 3,5.

Gen-ID: 70747

UniProt: Q922J6