TMEM175 Antikörper (Loop 4, Lumenal Region)

Produktnummer ABIN7886968

Der Kaninchen Polyklonal anti-TMEM175 Antikörper (ABIN7886968) detektiert spezifisch TMEM175 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für TMEM175 Antikörper (Loop 4, Lumenal Region) (ABIN7886968)

Target: TMEM175 (Transmembrane Protein 175 (TMEM175))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TMEM175 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-TMEM175 Antikörper

Bindungsspezifität: AA 278-291, Loop 4, Lumenal Region

Verwendungszweck: A Rabbit Polyclonal antibody to TMEM175

Sequenz: CEDNVPDPKD VQEK

Homologie: Rat - 13 out of 14 amino acid residues identical Human - 12 out of 14 amino acid residues identical

Produktmerkmale: Anti-TMEM175 Antibody (ABIN7581993) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM175 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CEDNVPDPKDVQEK, corresponding to amino acid residues 278 - 291 of mouse TMEM175

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu TMEM175

Target: TMEM175 (Transmembrane Protein 175 (TMEM175))

Andere Bezeichnung: TMEM175

Hintergrund:

Synonyms: Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175

Description: Transmembrane protein 175, TMEM175, is an endosomal/lysosomal proton channel that plays a crucial role in preserving the lysosomal membrane potential and maintaining pH stability. TMEM175 mediates lysosomal proton efflux, known as the "H+ leak" pathway, preventing excessive acidification of lysosomes.1 Intracellular endosomes and lysosomes rely on a vacuolar-type H+ ATPase (V-ATPase) to establish a significant proton concentration gradient across their membranes, which plays a crucial role in facilitating active transport of ions and metabolites between the vesicular lumen and the cytosol. To effectively digest macromolecules, lysosomes rely on an acidic environment with a pH range of 4.5 to 5.0, which is maintained through the entry of protons by the V-ATPase pump and their exit through the "H+ leak" pathway, which is the passive release of H+ into the cytosol. TMEM175 functions as a proton-activated, proton-selective channel on the lysosomal membrane in order to mediate the "H+ leak" pathway, prohibiting the excessive acidification of lysosomes. When lysosomes are acidified beyond the typical range, TMEM175 is activated, prohibiting further lysosomal acidification.1Mammalian TMEM175 consists of two homologous 6-TM domains and is suggested to function as a dimer. TM1 serves as the inner helix lining the pore and forms an hourglass-shaped ion permeation pathway within the channel. The C-terminal half of TM1 contains hydrophobic residues that create a bottleneck along the ion conduction pathway, serving as the selectivity filter of the channel. TMEM175 conducts K+ constitutively at pH 7.4, but shows reduced K+ permeation at lower pH . Conversely, proton current through TMEM175 increases with decreasing pH due to the increased proton gradient. Molecular dynamics simulation, structure-based mutagenesis, and electrophysiological analysis suggest that K+ ions and protons share the same permeation pathway in TMEM175.2,3Altered lysosomal pH disrupts various cellular processes, including lysosomal degradation, cargo loading, catabolite export, vesicle movement, and nutrient sensing. This impairment contributes to the pathogenesis of neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and lysosomal storage diseases. Optimization of lysosomal pH can effectively clear toxic protein aggregates associated with these diseases. Thus, maintaining lysosomal pH homeostasis plays a crucial role in lysosome function, cellular health, and neuropathology.1

Gen-ID: 72392

UniProt: Q9CXY1