PACC1 (AA 95-110), (Extracellular) Antikörper

Produktnummer ABIN7581942

Der Kaninchen Polyklonal anti-PACC1 Antikörper (ABIN7581942) detektiert spezifisch PACC1 in FACS, IHC, LCI und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für PACC1 (AA 95-110), (Extracellular) Antikörper (ABIN7581942)

Target: PACC1

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Western Blotting (WB)

Produktdetails

Bindungsspezifität: AA 95-110, Extracellular

Verwendungszweck: A Rabbit Polyclonal antibody to PACC1/TMEM206 (extracellular)

Sequenz: (C)KLKHPVMSVS YKEVDR

Spezifität: Extracellular loop.

Homologie: Rat,human - identical

Produktmerkmale: Anti-PACC1/TMEM206 (extracellular) Antibody (ABIN7581942) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PACC1 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KLKHPVMSVSYKEVDR, corresponding to amino acid residues 95 - 110 of mouse PACC1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Antigendetails

Target: PACC1

Hintergrund: Proton-Activated Chloride Channel, PAC, Transmembrane Protein 206, Proton Activated Chloride Channel 1, Acid-Sensitive Outwardly-Rectifying Anion Channel, ASOR,The proton-activated Cl- (PAC) channel, which opens by extracellular acidic pH , has been well characterized in several cells and tissues by electrophysiology recordings, although its molecular identity was unknown.Recently, two independent groups, identified a two-transmembrane protein named PACC1 (or TMEM206) as the molecular underlie of the proton-activated Cl- channel 1,2.PACC1 has no discernible sequence homology with previously characterized Cl- channels, although its structure of two transmembrane domains with cytoplasmic N- and C-termini and a large extracellular loop, resembles that of the Na+ -selective channels ASICs and ENaCs. In fact, recent work suggests that the functional unit of the proton-activated Cl- channel is a trimeric form of PACC1 subunits3,4.PAC activity that is stimulated by the lowering of extracellular pH and has been recorded in a wide range of mammalian cells and tissues. Acidic pH is crucial for the function of intracellular organelles in the secretory and endocytic pathways and is also one of the pathological hallmarks of many diseases, including cerebral and cardiac ischemia, cancer, infection and inflammation. Therefore, it is no surprise that the involvement of PACC1 in several physiological processes of the endocytic pathway5,6 and pathological conditions such as acid-induced cell death and brain damage after ischemic stroke7 or that upregulation of PACC1 is associated with poor prognosis in patients with hepatocellular carcinoma8.

Gen-ID: 66950

UniProt: Q9D771