NEGR1 Antikörper (Extracellular)

Produktnummer ABIN7581917

Der Kaninchen Polyklonal Anti-NEGR1-Antikörper wurde für WB, IHC, FACS und LCI validiert. Er ist geeignet, NEGR1 in Proben von Maus zu detektieren.

Kurzübersicht für NEGR1 Antikörper (Extracellular) (ABIN7581917)

Target: NEGR1 (Neuronal Growth Regulator 1 (NEGR1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEGR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-NEGR1 Antikörper

Bindungsspezifität: Extracellular

Verwendungszweck: A Rabbit polyclonal antibody to NEGR1 (extracellular).

Sequenz: (C)HISPSAKPFE NGQYLD

Spezifität: Extracellular. 

Homologie: Human,Rat - identical 

Produktmerkmale: Anti-NEGR1 (extracellular) Antibody (ABIN7581917) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NEGR1 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HISPSAKPFENGQYLD, corresponding to amino acid residues 168 -183 of mouse NEGR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NEGR1

Target: NEGR1 (Neuronal Growth Regulator 1 (NEGR1))

Andere Bezeichnung: NEGR1

Hintergrund: Neuronal Growth Regulator 1, IgLON Family Member 4, IGLON4 ,Neuronal growth regulator 1 (NEGR1), also known as neurotractin or Kilon, is a member of the immunoglobulin superfamily's IgLON subgroup. Structurally, IgLON proteins share three Ig-like C2-type domains and are anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor1,2. These proteins are implicated in neural development, supporting cellular interactions, neurite outgrowth, and synaptic formation3,4. NEGR1 is predominantly expressed in brain tissue, especially in the cerebral cortex, hippocampus, and hypothalamus. At the cellular level, it localizes to neuronal cell membranes where it regulates neurite extension and synapse stabilization1,5. NEGR1 activity is regulated through interaction with signaling molecules such as leukemia inhibitory factor receptor (LIFR), which affects downstream factors like lipocalin-2 (Lcn2) that are vital for neurogenesis and affective behaviors3. Functionally, NEGR1 modulates cell adhesion and neurite growth, processes essential for the proper development of neural circuits. It plays roles in both physiological and pathological states, for example, it is linked to hippocampal neurogenesis and synaptic plasticity, influencing behaviors related to anxiety and depression3,5. Dysfunctions in NEGR1 are associated with obesity, learning difficulties, psychiatric disorders, and mood dysregulation. Knockout studies in mice reveal that the loss of NEGR1 leads to reduced body mass, anxiety-like behaviors, and impaired neurogenesis2,4.

Gen-ID: 320840

Pathways: Feeding Behaviour