MRGPRX2 Antikörper (Extracellular)

Produktnummer ABIN7581916

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MRGPRX2 in WB, FACS und LCI. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MRGPRX2 Antikörper (Extracellular) (ABIN7581916)

Target: MRGPRX2 (G Protein-Coupled Receptor MRGX2 (MRGPRX2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MRGPRX2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-MRGPRX2 Antikörper

Bindungsspezifität: AA 5-19, Extracellular

Verwendungszweck: A Rabbit Polyclonal antibody to Human MRGPRX2 (extracellular)

Sequenz: (C)TPAWGTESTT VNGND

Spezifität: Extracellular, N-terminus.

Homologie: Human only No cross reactivity with mouse,rat related proteins

Produktmerkmale: Anti-Human MRGPRX2 (extracellular) Antibody (ABIN7581916) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize the human MRGPRX2 protein. It won't recognize any mouse or rat related proteins.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)TPAWGTESTTVNGND, corresponding to amino acid residues 5 - 19 of human MRGPRX2 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu MRGPRX2

Target: MRGPRX2 (G Protein-Coupled Receptor MRGX2 (MRGPRX2))

Andere Bezeichnung: MRGPRX2

Hintergrund: Mas-related G-protein coupled receptor member X2, MRGX2,MRGPRX2, also known as Mas-related G protein-coupled receptor member X2, is a member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily and the Mas-related subfamily branch of the rhodopsin-like class A GPCRs or Mas-related G protein-coupled receptors (Mrgprs).1,2Mrgprs first described 25 years ago, comprise more than 50 receptors in humans and rodents, most of which are still considered orphan receptors, that is, receptors with no identified endogenous or synthetic ligands.1.2The MRGPRX subfamily consists of four proteins (MRGPRX1 to MRGPRX4). The four proteins have sequence similarity with the MrgA Mas-related subfamily, however, the MRGPRX genes are only present in primates with no true orthologues in mice or rats. 1,2MRGPRX2 highest expression is observed in mast cells, although it has also been identified in other peripheral blood cells, including basophils and eosinophils, and at low levels in other tissues, such as the gastrointestinal tract and the central nervous system.1.2The expression and function of MRGPRX2 in mast cells garnered considerable interest, as these cells play a crucial role in the immune system and are involved in allergic reactions and inflammation. Indeed, MRGPRX2 activation in mast cells, triggers a signaling pathway that leads to the release of various inflammatory mediators, such as histamine, cytokines, and chemokines. This release of mediators contributes to the initiation and amplification of inflammatory responses. 3-5The mast cell-mediated classical allergic response, involves crosslinking of high affinity immunoglobulin E (IgE) receptor (FcεRI) on mast cells with allergen, which then release a wide range of pre-stored inflammatory mediators (histamine, serotonin, protease), followed by de novo synthesis of mediators (prostaglandins and leukotrienes) and a variety of cytokines such as interleukin (IL)-4, IL-5, IL-9, and IL-13. 3-5MRGPRX2 has been linked to pseudo allergic reactions, which mimic true allergic reactions but do not involve IgE antibodies. Pseudo allergic reactions can be triggered by substances that directly activate MRGPRX2 on mast cells, leading to the release of histamine and other inflammatory mediators.3-5In addition, expression of MRGPRX2 in sensory neurons, may contribute to the sensation of itch and pain, thus highlighting its role in neurogenic inflammation beyond classical allergic responses.3-5

Gen-ID: 117194

UniProt: Q96LB1