MEGF10 Antikörper (Extracellular)

Produktnummer ABIN7581914

Der Kaninchen Polyklonal Anti-MEGF10-Antikörper wurde für WB, IHC, FACS und LCI validiert. Er ist geeignet, MEGF10 in Proben von Maus zu detektieren.

Kurzübersicht für MEGF10 Antikörper (Extracellular) (ABIN7581914)

Target: MEGF10 (Multiple EGF-Like-Domains 10 (MEGF10))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEGF10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-MEGF10 Antikörper

Bindungsspezifität: AA 178-190, Extracellular

Verwendungszweck: A Rabbit Polyclonal antibody to MEGF10 (extracellular)

Sequenz: CEDR(S)EQGTYGND

Spezifität: Extracellular, N-terminus.

Kreuzreaktivität (Details): This antibody is specific for MEGF10, it won't recognize the closely related protein MEGF11 (Accession Q80T91)

Homologie: Mouse,rat,human - 12 out of 13 amino acid residues identical

Produktmerkmale: Anti-MEGF10 (extracellular) Antibody (ABIN7581914) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize MEGF10 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CEDR(S)EQGTYGND, corresponding to amino acid residues 178 - 190 of mouse MEGF10

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu MEGF10

Target: MEGF10 (Multiple EGF-Like-Domains 10 (MEGF10))

Andere Bezeichnung: MEGF10

Hintergrund: Multiple EGF Like Domains 10, Multiple EGF-Like Domains Protein 10,MEGF10 is a member of the multiple epidermal growth factor-like domains protein family. MEGF10 is a type I transmembrane protein that consists of 15 EGF-like domains in its extracellular region and is the mammalian homolog of Draper (a Drosophila phagocytosis apoptotic cell receptor)1.MEGF10 is a mediator for apoptotic cell phagocytosis by central nervous system (CNS) macrophages- microglia and astrocytes, through binding with high affinity to C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells2. Also necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum2. In this regard, MEGF10 works alongside other phagocytic receptors expressed in astrocytes or microglia such as the tyrosine kinase receptors MERTK and AXL, the G-protein coupled receptors Bai1 and GPR56 or the TREM2 receptor3.MEGF10 is involved in the uptake of amyloid-β peptide (Aβ42) in the brain. Alzheimer's disease (AD) is characterized by extracellular accumulation of senile plaques, the major component of which is the amyloid-β peptide (Aβ), and the presence of neurofibrillary tangles. MEGF10 as a functional receptor that mediates the uptake of amyloid-β peptide will help elucidate the molecular mechanisms of amlyoid-β clearance in Alzheimer's disease1,4.MEGF10 is also an essential factor in the regulation of myogenesis. It controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway5. In fact, mutations in the MEGF10 gene are the underlying cause of Early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD), a lethal congenital myopathy characterized by hypotonia with respiratory distress 5,6.

Gen-ID: 70417

UniProt: Q6DIB5

Pathways: Regulation of Muscle Cell Differentiation