GPR160 Antikörper (Intracellular)

Produktnummer ABIN7581887

Der Kaninchen Polyklonal Anti-GPR160-Antikörper wurde für WB und IHC validiert. Er ist geeignet, GPR160 in Proben von Ratte zu detektieren.

Kurzübersicht für GPR160 Antikörper (Intracellular) (ABIN7581887)

Target: GPR160 (G Protein-Coupled Receptor 160 (GPR160))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR160 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GPR160 Antikörper

Bindungsspezifität: AA 122-136, Intracellular

Verwendungszweck: A Rabbit Polyclonal antibody to GPR160

Sequenz: CNLSRATKPS SRWQK

Spezifität: Intracellular, 2nd loop.

Homologie: Mouse - 13 out of 15 amino acid residues identical It won't recognize human samples

Produktmerkmale: Anti-GPR160 Antibody (ABIN7581887) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR160 from rat and mouse samples. The antibody will not recognize human GPR160.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CNLSRATKPSSRWQK, corresponding to amino acid residues 122 - 136 of rat GPR160

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR160

Target: GPR160 (G Protein-Coupled Receptor 160 (GPR160))

Andere Bezeichnung: GPR160

Hintergrund: G-Protein Coupled Receptor 160,G-protein coupled receptor 160, GP160 or GPR160, is an orphan class A GPCR that plays a role in pain signaling.1,3GPR160 is composed of 338 amino acids, encoded by 7 exons located at 3q26.2-q27, demonstrates a significant degree of conservation across various species, and is expressed in neurons, astrocytes, and microglia within the central nervous system (CNS) and spinal cord.3Cocaine- and amphetamine-regulated transcript peptide (CARTp) exhibits various pharmacological actions in the CNS, peripheral nervous system, and several endocrine organs. It plays a role in reward and addiction, anxiety and depression, and food intake and body weight maintenance. It has been found that the interaction between CARTp and GPR160 is essential for the circadian regulation of appetite and thirst as well as the transmission of pain signals induced by nerve injury. GPR160 has been shown to confer CARTp-induced cFOS and ERK phosphorylation and activation of CREB independently of cAMP signaling. ERK can function as an upstream regulator of CREB phosphorylation during the development of neuropathic pain. As a putative ligand for GPR160, endogenous CARTp inhibition has been shown to mitigate neuropathic pain.1,2GPR160 has also been shown to play a role in the pathogenesis of prostate cancer. Transcription profiles demonstrate a marked increase of GPR160 mRNA in all stages of prostate cancer samples and cancer cell lines, except prostate hyperplasia tissues, with levels comparable to those in stage II-IV cancer patients. GPR160 knockdown increased caspase 1 and IL-6 expression and induced cell cycle arrest and apoptosis. The involvement of GPR160 in prostate carcinogenicity has therapeutic implications for exploring diagnostics and pharmaceutical interventions.3

Gen-ID: 499588

UniProt: Q66H29