GPR143 Antikörper (Intracellular, Loop 3)

Produktnummer ABIN7886465

Der Kaninchen Polyklonal anti-GPR143 Antikörper (ABIN7886465) detektiert spezifisch GPR143 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für GPR143 Antikörper (Intracellular, Loop 3) (ABIN7886465)

Target: GPR143 (G Protein-Coupled Receptor 143 (GPR143))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR143 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GPR143 Antikörper

Bindungsspezifität: AA 225-239, Intracellular, Loop 3

Verwendungszweck: A Rabbit Polyclonal antibody to GPR143

Sequenz: (C)KGRKGVYTEN ERLMG

Homologie: Rat- identical, human - 12 out of 15 amino acid residues identical

Produktmerkmale: Anti-GPR143 Antibody (ABIN7581885) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR143 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KGRKGVYTENERLMG, corresponding to amino acid residues 225 - 239 of mouse GPR143

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400-1:500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR143

Target: GPR143 (G Protein-Coupled Receptor 143 (GPR143))

Andere Bezeichnung: GPR143

Hintergrund:

Synonyms: G-Protein Coupled Receptor 143, Ocular Albinism Type 1 Protein, OA1

Description: G-protein coupled receptor 143, GPR143, also known as ocular albinism type 1 protein, OA1, is an orphan GPCR expressed intracellularly in pigment-producing cells on the melanosomal membrane and serves to regulate melanosome size and mediate protein transport to the melanosome via the endolysosomal system.1GPR143 is encoded by the OA1 gene, composed of 404 amino acids with 7 transmembrane helices, and associates with several Gα and Gβ subunits, as well β-Arrestin. Potential ligands for GPR143 include L-3,4-dihydroxyphenylalanine (L-DOPA) and dopamine. High dose L-DOPA treatment leads to intracellular Ca2+ influx and β-Arrestin recruitment. GPR143 has two sorting signals, a dileucine motif in ICL3 and a tryptophan-glutamic acid doublet in the C-terminal tail, which are necessary for lysosomal and melanosomal localization. Functional components of endosomal sorting complexes required for transport (ESCRT) are essential for intracellular sorting and ubiquitination. Due to the unique localization of GPR143 within the cell and its topological orientation, ligands are likely required to bind from the organelle lumen.1GPR143 is largely expressed on the melanosomal membrane of melanocytes, the pigment producing cells that are responsible for producing melanin in the skin and eyes. Melanin is a pigment that is crucial for the protection of skin and eyes from damaging UV light, as well as for the normal development of the optic system and eye function. Mutations in the OA1 gene and its subsequent protein products lead to ocular albinism due to melanosome malformation. Albinism is a pigment disorder characterized by decreased melanin in affected tissues, which leads to adverse effects including foveal hypoplasia and chiasmal misrouting.1,3GPR143 pathophysiology is also implicated in various degenerative diseases, including macular degeneration and Parkinson's disease, as well as playing a possible role in cancer susceptibility and progression.1,2

Gen-ID: 18241

UniProt: P70259