G Protein-Coupled Receptor 12 Antikörper (Extracellular)

Produktnummer ABIN7581884

Der Kaninchen Polyklonal Anti-G Protein-Coupled Receptor 12-Antikörper wurde für WB und IHC validiert. Er ist geeignet, G Protein-Coupled Receptor 12 in Proben von Maus zu detektieren.

Kurzübersicht für G Protein-Coupled Receptor 12 Antikörper (Extracellular) (ABIN7581884)

Target: G Protein-Coupled Receptor 12 (GPR12)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser G Protein-Coupled Receptor 12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-G Protein-Coupled Receptor 12 Antikörper

Bindungsspezifität: AA 3-15, Extracellular

Verwendungszweck: A Rabbit Polyclonal antibody to SORCS2

Sequenz: (C)EDPKVNLSGL PRD

Spezifität: Extracellular, N-term.

Homologie: Rat - identical,Human - 12 out of 13 amino acid residues identical

Produktmerkmale: Anti-GPR12 (extracellular) Antibody (ABIN7581884) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR12 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)EDPKVNLSGLPRD, corresponding to amino acid residues 3 - 15 of mouse GPR12 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu G Protein-Coupled Receptor 12

Target: G Protein-Coupled Receptor 12 (GPR12)

Andere Bezeichnung: GPR12 

Hintergrund: G-Protein Coupled Receptor 12, GPCR21,G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs is a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR12 is an orphan G protein-coupled receptor related phylogenetically, functionally, and pharmacologically with the cannabinoid system. It considered as a "constitutively active receptor" since it greatly stimulates adenylate cyclase in the absence of ligand-receptor interaction. GPR12 seems to couple Gαs as well as Gαi proteins since it stimulates baseline cAMP-dependent signaling by activating Gαs proteins, and also inhibits forskolin activated signaling through Gαi interaction3.GPR12 expressed in the pituitary gland, in neurons in the frontal cortex, piriform cortex, thalamus, hypothalamus, hippocampus, amygdala, and olfactory bulb. It was found that GPR12 plays a significant role in energy balance. GPR12 knock out mice exhibit increase body weight and body fat mass. In addition, overexpression of GPR12 leads to cancer cells migration and invasion via phosphorylation of Keratin 84.Furthermore, GPR12 has been identified as an inverse agonist of the phytocannabinoid cannabidiol (CBD)4.

Gen-ID: 14738

UniProt: P35412