GAP43 Antikörper (AA 205-218)

Produktnummer ABIN7581879

Dieses Anti-GAP43-Antikörper ist ein Meerschweinchen Polyklonal-Antikörper zur Detektion von GAP43 in WB und IHC. Geeignet für Ratte.

Kurzübersicht für GAP43 Antikörper (AA 205-218) (ABIN7581879)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser GAP43 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GAP43 Antikörper

Bindungsspezifität: AA 205-218

Verwendungszweck: A Guinea Pig Polyclonal Antibody to GAP43

Sequenz: (C)KPKESARQDE GKED

Spezifität: C-terminus

Homologie: Mouse - identical, human - 13 out of 14 amino acid residues identical

Produktmerkmale: Guinea Pig Anti-GAP43 Antibody (ABIN7581879) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-GAP43 Antibody (ABIN7043263, ABIN7044662 and ABIN7044663) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7235735)

Blocking Peptide: (ABIN7235735)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Andere Bezeichnung: GAP43

Hintergrund: Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50,Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Gen-ID: 29423

UniProt: P07936