CALHM2 Antikörper (Extracellular)

Produktnummer ABIN7581840

Produktdetails anti-CALHM2 Antikörper

Target: CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))

Bindungsspezifität: AA 133-146, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CALHM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC)

Verwendungszweck: A Rabbit Polyclonal antibody to CALHM2 (extracellular)

Sequenz: (C)SEFVDPSSLT AGDK

Spezifität: Extracellular, 2nd loop.

Homologie: Rat - 13 out of 14 amino acid residues identical Human - 11 out of 14 amino acid residues identical

Produktmerkmale: Anti-CALHM2 (extracellular) Antibody (ABIN7581840) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CALHM2 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)SEFVDPSSLTAGDK, corresponding to amino acid residues 133 - 146 of mouse CALHM2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CALHM2

Target: CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))

Andere Bezeichnung: CALHM2 (CALHM2 Produkte)

Hintergrund: Calcium homeostasis modulator protein 2, Protein FAM26B, FAM26B,Calcium homeostasis modulator protein 2 (CALHM2) is a member of the CALHM channel family, a group of transmembrane proteins that play essential roles in calcium ion transport and homeostasis. The CALHM family consist in humans of six members: CALHM1 to CALHM6.1CALHM2 functions as a calcium-permeable ion channel. It allows calcium ions to pass through the cell membrane in response to specific stimuli, such as changes in membrane potential or extracellular calcium concentration.2,3CALHM2 is composed of 323 amino acids and assembles as an undecamer with each protomer containing a large N-terminal transmembrane (TM) domain with 4 TM helices, an intracellular C-terminal domain, and a small extracellular linker region. 2,3CALHMs permeate ions and ATP in a voltage-dependent manner to modulate neuronal excitability. CALHM2 has been shown to accommodate lipids in its hydrophobic channel in order to regulate channel function.2,3Ion and ATP permeation by CALHM proteins play a role in the physiology of cognition, depression, gustatory signaling, and the pathology of Alzheimer's disease.Specifically, CALHM2 expression was increased in Alzheimer's disease (AD) patients and in an AD model in mice. Knockout of CALHM2 improved Aβ plaque deposition, reduced neuroinflammation, and improved cognitive impairment in an AD mice model.4Moreover, microglial CALHM2 may have a central role in both chronic inflammatory diseases (like AD) and in acute inflammatory reactions and neuroinflammation.4

Gen-ID: 72691

UniProt: Q8VEC4