ATP1A1 Antikörper (Intracellular)

Produktnummer ABIN7581827

Der Meerschweinchen Polyklonal Anti-ATP1A1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, ATP1A1 in Proben von Ratte zu detektieren.

Kurzübersicht für ATP1A1 Antikörper (Intracellular) (ABIN7581827)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser ATP1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ATP1A1 Antikörper

Bindungsspezifität: AA 7-20, Intracellular

Verwendungszweck: A Guinea Pig Polyclonal Antibody to Alpha 1 Na+/K+ ATPase

Sequenz: (C)RDKYEPAAVS EHGD

Spezifität: Intracellular, N-terminus

Homologie: Mouse,dog,pig - identical,Human- 13 out of 14 amino acid residues identical

Produktmerkmale: Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody (ABIN7581827) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Alpha 1 Na+/K+ ATPase Antibody (ABIN7043659, ABIN7044637 and ABIN7044638) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7236890)

Blocking Peptide: (ABIN7236890)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Andere Bezeichnung: ATP1A1

Hintergrund: ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1,Na+/K+-ATPase, a membrane protein located on the plasma membrane of all animal cells, is involved in maintaining the Na+ and K+ gradients across the cell membrane1. It is ubiquitously expressed in neurons and helps maintain normal neuronal function2. The enzyme is composed of a catalytic α chain of 112 kDa. and α glycosylated β chain of 40-50 kDa. There are four isoforms of the α subunit (α1, α2, α3 and α4) and three isoforms of the β subunit (β1, β2 and β3) with different tissue distributions3. The N-terminus is located on the cytoplasmic side of the membrane. There are four transmembrane segments in the first third of the polypeptide chain, a large cytoplasmic loop (LCL) comprising the second third of the chain and four to six transmembrane segments in the C-terminal third of the protein with the C-terminus also on the cytoplasmic side of the membrane4. Na+/K+-ATPase abnormalities have been reported to be involved in several neurological diseases such as seizures, bipolar disorder, spongiform encephalopathy, and Alzheimer's disease. Na+/K+-ATPase may also have implications in behavioral defects5.

Gen-ID: 24211

UniProt: P06685

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process