Aquaporin 4 Antikörper (Intracellular)

Produktnummer ABIN7581826

Dieses Meerschweinchen Polyklonal-Antikörper erkennt spezifisch Aquaporin 4 in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für Aquaporin 4 Antikörper (Intracellular) (ABIN7581826)

Target: Aquaporin 4 (AQP4)

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser Aquaporin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Aquaporin 4 Antikörper

Bindungsspezifität: AA 300-314, Intracellular

Verwendungszweck: A Guinea Pig Polyclonal Antibody to Aquaporin 4 (AQP4)

Sequenz: (C)HVIDIDRGDE KKGKD

Spezifität: Intracellular, C-terminus

Homologie: human - 13,15 amino acid residues identical,Mouse - 14

Produktmerkmale: Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody (ABIN7581826) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Aquaporin 4 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Aquaporin 4 (AQP4) (300-314) (ABIN7042939, ABIN7045209 and ABIN7045210) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200-1:300

Application Dilutions Western blot wb: 1:400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Aquaporin 4

Target: Aquaporin 4 (AQP4)

Andere Bezeichnung: AQP4

Hintergrund: WCH4, Mercurial-insensitive water channel, MIWC,Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.

Gen-ID: 25293

UniProt: P47863

Pathways: Sensory Perception of Sound