Apelin Receptor Antikörper (Extracellular)

Produktnummer ABIN7581824

Der Kaninchen Polyklonal Anti-Apelin Receptor-Antikörper wurde für WB, IHC, IF und FACS validiert. Er ist geeignet, Apelin Receptor in Proben von Ratte zu detektieren.

Kurzübersicht für Apelin Receptor Antikörper (Extracellular) (ABIN7581824)

Target: Apelin Receptor (APLNR)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Apelin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Apelin Receptor Antikörper

Bindungsspezifität: AA 4-17, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Apelin Receptor

Sequenz: DGYNYYGADN QSEC

Spezifität: Extracellular, N-terminus

Homologie: Mouse,human - identical

Produktmerkmale: Anti-Apelin/APJ Receptor (extracellular) Antibody (ABIN7581824) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Apelin receptor from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Apelin Receptor

Target: Apelin Receptor (APLNR)

Andere Bezeichnung: APLNR

Hintergrund: APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34,The apelin receptor (APJ) is a member of the G-protein coupled receptor gene family, encoded by the APLNR gene. The apelin receptor and its endogenous ligand, apelin, have been suggested to act as key mediators of physiological responses to multiple homeostatic actions, including cardiovascular control, water balance, hypothalamic-pituitary-adrenal (HPA) axis regulation and metabolic homeostasis.Apelin receptor structure contains seven hydrophobic transmembrane domains, with several sites for phosphorylation by protein kinase A, and sites for palmitoylation and glycosylation. The N-terminal glycosylation is implicated in the receptor's expression, stability, correct folding of the protein and ligand binding. The C-terminal palmitoylation has been reported to play a role in membrane association. These modifications can influence the internalization, dimerization and ligand binding of the receptor. Human apelin receptor shares 92 % homology with mouse receptor and 90 % homology with rat receptor.Apelin expression can be detected in several brain regions including the human caudate nucleus, corpus callosum, hippocampus, substantia nigra, subthalamic nucleus, medulla and spinal cord. Apelin receptor is also expressed in the spleen, placenta and cardiovascular tissues and small amounts in the small intestine, lung, stomach, colonic mucosa and ovary.

Gen-ID: 83518

UniProt: Q9JHG3