ADORA1 Antikörper (Extracellular, Loop 2)

Produktnummer ABIN7886047

Der Kaninchen Polyklonal anti-ADORA1 Antikörper (ABIN7886047) detektiert spezifisch ADORA1 in WB, IHC, FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für ADORA1 Antikörper (Extracellular, Loop 2) (ABIN7886047)

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADORA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-ADORA1 Antikörper

Bindungsspezifität: AA 153-168, Extracellular, Loop 2

Verwendungszweck: A Rabbit Polyclonal antibody to Adenosine A1 Receptor (extracellular)

Sequenz: (C)EQDWRANGSV GEPVIK

Homologie: Mouse - 14 out of 16 amino acid residues identical Not recommended for human samples

Produktmerkmale: Anti-Adenosine A1 Receptor (extracellular) Antibody (ABIN7581822) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Adenosine A1 Receptor from mouse and rat samples. This antibody is not recommended for human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)EQDWRANGSVGEPVIK, corresponding to amino acid residues 153 - 168 of rat Adenosine A1 Receptor 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Andere Bezeichnung: ADORA1

Hintergrund:

Synonyms: Adenosine Receptor A1, Adenosine A1R, ADORA1

Description: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3A1AR is widely distributed and has been well characterized. High expression of A1AR is found in the brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, the colon, and kidney.2,4A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8,9

Gen-ID: 29290

UniProt: P25099

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane