Double-Stranded RNA (dsRNA) Antikörper

Produktnummer ABIN7567105

Dieser Maus Monoklonal Antikörper detektiert spezifisch Double-Stranded RNA in ELISA und IF.

Kurzübersicht für Double-Stranded RNA (dsRNA) Antikörper (ABIN7567105)

Target: Double-Stranded RNA (dsRNA)

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Klon: K2

Produktdetails

Verwendungszweck: Mouse anti double-stranded RNA (K2)

Spezifität: The mAb K2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (4050 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by K2. As described by Schönborn et al. K2 binds with high avidity to all dsRNAs investigated.

Produktmerkmale: K2 anti-dsRNA monoclonal antibody has an IgM isotype. K2 has primarily been used in ELISA and sandwich ELISA. Generally, K2 can be used in applications where an anti-dsRNA antibody with an isotype other than IgG (IgG2a) is required.The mAb K2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (4050 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by K2. As described by Schönborn et al. K2 binds with high avidity to all dsRNAs investigated.

Aufreinigung: Undiluted hybridoma supernatant

Immunogen: Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: MAb K2 is primarily used for a sandwich ELISA to detect and quantitate (after calibration) dsRNA (see Schönborn et al.). For this application it should be diluted with PBS. It may also be advantageous to use K2 for immunofluorescence studies. The optimum working dilution of the antibody for any specific application should be established by titration. Not for use for clinical purposes. For in vitro use only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Culture supernatant (RPMI, 5% fetal calf serum). No preservative is added.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: After delivery antibodies should be aliquoted and stored at -20 ° or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the antibody should be kept frozen. Repeated freezing/thawing cycles should be avoided.

Antigendetails

Target: Double-Stranded RNA (dsRNA)

Andere Bezeichnung: dsRNA

Substanzklasse: Nucleotide

Hintergrund: Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010).