Rekombinanter TNFSF13 Antikörper

Produktnummer ABIN7566472

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TNFSF13 in ELISA, IP und Func. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Rekombinanter TNFSF13 Antikörper (ABIN7566472)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNFSF13 Antikörper ist unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Klon: Apry-1-1

Produktdetails anti-TNFSF13 Antikörper

Verwendungszweck: anti-APRIL (mouse), mAb (rec.) (blocking) (Apry-1-1) (Fc LAEA-PG)

Produktmerkmale:

Recombinant Antibody. Recognizes mouse APRIL. Does not recognize human APRIL and mouse/human BAFF. Source: HEK293. Isotype: Mouse IgG2blambda. Immunogen: Mouse APRIL (aa 98-232). Applications: ELISA, FUNC (Blocking), IP. Inhibits the binding of mouse APRIL to BCMA and TACI. Works in vitro and in vivo. APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. anti-APRIL (mouse), mAb (rec.) (Blocking) (Apry-1-1) (Fc LAEA-PG) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc mouse portion with mutations LAEA-PG and then produced in mammalian cells to generate an IgG like scFv-Fc (LAEA-PG) fusion protein. Mutations LAEA-PG inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected.

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. anti-APRIL (mouse), mAb (rec.) (Blocking) (Apry-1-1) (Fc LAEA-PG) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc mouse portion with mutations LAEA-PG and then produced in mammalian cells to generate an IgG like scFv-Fc (LAEA-PG) fusion protein. Mutations LAEA-PG inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected.

Endotoxin-Niveau: <0.001EU/μg purified protein (LAL-test, Lonza)

Immunogen: Mouse APRIL (aa 98-232).

Isotyp: IgG2b lambda

Biological Activity Comment: Inhibits the binding of mouse APRIL to BCMA and TACI. Works in vitro and in vivo.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS.

Handhabung: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Stable for at least 1 month after receipt when stored at +4°C.

Stable for at least 1 year after receipt when stored at -20°C.

Details zu TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Andere Bezeichnung: APRIL

UniProt: Q9D777

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response