Collagen Type I Antikörper

Produktnummer ABIN7566413

Der Kaninchen Polyklonal Anti-Collagen Type I-Antikörper wurde für WB und ICC validiert. Er ist geeignet, Collagen Type I in Proben von Human, Ratte, Maus, Rind (Kuh), Hund, Schwein, Schaf, Cat und Hamster zu detektieren.

Kurzübersicht für Collagen Type I Antikörper (ABIN7566413)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund, Schwein, Schaf, Cat, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Produktdetails anti-Collagen Type I Antikörper

Verwendungszweck: anti-Collagen Type 1 (3/4 fragment), pAb

Produktmerkmale:

Polyclonal Antibody. Recognizes rat and bovine collagen type 1. Based on sequence identity should also recognize human, mouse, guinea pig, dog, cat, donkey, pig, cow, sheep and chicken collagen type 1. Source: Rabbit. Applications: ICC. Liquid. In PBS with 1 mg/mL BSA and 0.02 % sodium azide. The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis. Triple helical type I collagens are made up of two alpha1 (I) and one alpha2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components. Initial MMP-8 dependent cleavage of collagen into the characteristic 3/4 and 1/4 fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen 3/4 fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.

The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis. Triple helical type I collagens are made up of two alpha1 (I) and one alpha2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components. Initial MMP-8 dependent cleavage of collagen into the characteristic 3/4 and 1/4 fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen 3/4 fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.

Aufreinigung: Antigen affinity purified.

Immunogen: Synthetic peptide (human sequence) corresponding to the C-terminal end of the N-terminal three quarter collagen fragment (Col1 3/4).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: In PBS with 1 mg/mL BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Details zu Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Andere Bezeichnung: Collagen Type 1