KTI3 Antikörper (HRP)

Produktnummer ABIN7565993

Der Kaninchen Polyklonal anti-KTI3 Antikörper wird verwendet zum Nachweis von KTI3 in Proben von Lima bean. Er wurde validiert für WB, DB und IP.

Kurzübersicht für KTI3 Antikörper (HRP) (ABIN7565993)

Target: KTI3

Reaktivität: Lima bean

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), Dot Blot (DB), Immunoprecipitation (IP)

Produktdetails

Verwendungszweck: Trypsin Inhibitor Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Produktmerkmale: Synonyms: rabbit anti-trypsin inhibitor antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Aufreinigung: Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Trypsin Inhibitor [Lima Bean]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Trypsin Inhibitor Peroxidase Conjugated Antibody has been tested by Dot blot and is suitable to be assayed against 1.0 μg of Trypsin Inhibitor [Lima Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Immunoprecipitation Dilution: 1:100 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: KTI3

Hintergrund: Background: Trypsin Inhibitors are chemicals that reduce the availability of biologically active trypsin, an enzyme essential to nutrition of many animals, including humans. Tumor Associated Trypsin Inhibitor (TATI) has been used as a marker for various carcinomas and useful in cancer diagnosis.

Gen-ID: 547831

UniProt: P01070