Transferrin Antikörper (FITC)

Produktnummer ABIN7565982

Dieses Anti-Transferrin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Transferrin in WB, ELISA, DB, FM und FLISA. Geeignet für Human.

Kurzübersicht für Transferrin Antikörper (FITC) (ABIN7565982)

Target: Transferrin (TF)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Transferrin Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Produktdetails anti-Transferrin Antikörper (FITC)

Verwendungszweck: Transferrin Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Transferrin (Human Serum).

Produktmerkmale: Synonyms: rabbit anti-Transferrin Antibody Fluorescein Conjugated, Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody

Aufreinigung: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Transferrin (Human Serum)

Immunogen Type: Native Protein

Labeling Ratio: 4.5

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human transferrin Fluorescein has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Western Blot Dilution: User Optimized FLISA Dilution: User Optimized ELISA Dilution: 1:10,000 - 1:20,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Transferrin

Target: Transferrin (TF)

Andere Bezeichnung: Transferrin

Hintergrund: Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Gen-ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis