Pol (C-Term) Antikörper

Produktnummer ABIN7565974

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Pol in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für Pol (C-Term) Antikörper (ABIN7565974)

Target: Pol

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails

Bindungsspezifität: C-Term

Verwendungszweck: Orf2 Antibody

Kreuzreaktivität (Details): Anti-Orf2 is directed against mouse ORF2 near a c-terminus position.

Produktmerkmale: Synonyms: Rabbit Anti-Orf2 Antibody, LINE-1 retrotransposable element ORF2 protein, Endonuclease reverse transcriptase, Line1 protein, Orf2p, Retrovirus-related Pol polyprotein LINE-1, Long interspersed element-1

Aufreinigung: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-ORF2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminus of mouse ORF2 protein.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: This affinity purified antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~150 kDa in size corresponding to Orf2 by western blotting in the liver cell lysates or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000-1:150,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.07 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: Pol

Substanzklasse: Viral Protein

Hintergrund: Background: Anti-Orf2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). The multi-functional nuclear protein ORF2p is involved in reverse transcriptase and endonuclease processes. ORF2 elements are epigenetically silenced throughout embyronic development and it is suggested that mutations of L1 would result in detrimental de novo insertions into the genome, negatively affecting gene expression.  L1 elements are critical components in the mammalian genome, and much is still not known regarding these proteins. Studies implicate that line-1 is activated in fetal oocytes and  L1ORF2p may contribute to the eradication or selection of the oocytes.

Gen-ID: 2731767