HSF1 Antikörper (N-Term)

Produktnummer ABIN7565973

Dieses Anti-HSF1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HSF1 in WB und ELISA. Geeignet für Human.

Kurzübersicht für HSF1 Antikörper (N-Term) (ABIN7565973)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-HSF1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: HSF1 Antibody

Kreuzreaktivität (Details): Anti-HSF1 is directed against human HSF1 at a n-terminus position.

Produktmerkmale: Synonyms: rabbit anti-HSF1 antibody, HSF-1, HSF 1, heat shock factor protein 1, HSTF1, HSTF 1, HSTF-1, heat shock transcription factor 1

Aufreinigung: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-HSF1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the n-terminal portion of human HSF1 protein.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Heat Shock Factor 1 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of 57 kDa in size corresponding to HSF1 by western blotting in the appropriate cell lysate or extract. Tested using positive control A431 Whole Cell Lysate p/n W09-000-361. Western Blot Dilution: 1 μg/mL ELISA Dilution: 5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.96 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: HSF1

Hintergrund: Background: Anti-HSF1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Heat shock factor 1 (HSF1) is a highly conserved transcription factor that coordinates stress-induced transcription and directs versatile physiological processes in eukaryotes. Upon stress, HSF1 is the key transcriptional activator of chaperones, co-chaperones and ubiquitin, and also coordinates the expression of many transcriptional and translational regulators, signaling molecules and mitotic determinants. HSF1 is involved in balancing core cellular processes during stress and enables their rapid re-establishment once conditions suitable for proliferation have been restored. Importantly, HSF1 controls a distinct set of target genes in cell stress, development and cancer progression. The central role of HSF1 in diverse cellular functions is reflected in pathologies, such as neurodegenerative diseases and cancer, where an imbalance in HSF1 activity facilitates disease onset. The ability of cancer cells to harness HSF1 for metastatic progression highlights the plasticity of HSF1 in rewiring transcription and coordinating cellular processes. Anti-HSF1 antibody is thus ideal for investigators involved in cancer research and neurodegenerative diseases.

Gen-ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613