LacZ Antikörper

Produktnummer ABIN7565955

Dieses Anti--Antikörper ist ein Huhn Polyklonal-Antikörper zur Detektion von in ELISA, WB und DB. Geeignet für E. coli.

Kurzübersicht für LacZ Antikörper (ABIN7565955)

Target: LacZ

Reaktivität: E. coli

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB), Dot Blot (DB)

Produktdetails

Verwendungszweck: Beta Galactosidase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.

Produktmerkmale: Synonyms: chicken anti-Beta Galactosidase Antibody, chicken anti-Beta Gal Antibody, Beta Galactosidase [E.coli], BGAL, beta-galactosidase, beta-gal

Aufreinigung: This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Beta-Gal (CHICKEN) Antibody has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Western Blot Dilution: 1:2,000-1:10,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: LacZ

Hintergrund: Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

NCBI Accession: NP_414878

UniProt: P00722