EFNB1/EFNB2/EFNB3 (C-Term) Antikörper

Produktnummer ABIN7565951

Der Kaninchen Polyklonal anti-EFNB1/EFNB2/EFNB3 Antikörper wird verwendet zum Nachweis von EFNB1/EFNB2/EFNB3 in Proben von Human. Er wurde validiert für ELISA, FM, IF und WB.

Kurzübersicht für EFNB1/EFNB2/EFNB3 (C-Term) Antikörper (ABIN7565951)

Target: EFNB1/EFNB2/EFNB3

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Fluorescence Microscopy (FM), Immunofluorescence (IF), Western Blotting (WB)

Produktdetails

Bindungsspezifität: C-Term

Verwendungszweck: Ephrin B1, B2, B3 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Ephrin B1, B2, B3.

Produktmerkmale: Synonyms: Rabbit Anti-Ephrin B1, B2, B3 Antibody, Ephrin-B1, Ephrin-B2, Ephrin-B3, ELK Ligand, EPLG2, LERK2, EFL3, Craniofrontonasal Syndrome (Craniofrontonasal Dysplasia), EPH-Related Receptor Transmembrane Ligand ELK-L3, Eph-Related Receptor Tyrosine Kinase Ligand 2, Eph-Related Receptor Tyrosine Kinase Ligand 8, EPH-Related Receptor Tyrosine Kinase Ligand 2, LERK-2, LERK-8, Elk-L, EFL-3, ELK-L, CFND, EFL6, EFB1, CFNS

Aufreinigung: This affinity purified antibody is directed against human Ephrin B1, B2, B3.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Ephrin B1, B2, B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Ephrin B1, B2, B3 Antibody has been tested in ELISA, Western Blot, and Immunofluorescence. Expect a band at ~35-38 kDa in western blot using appropriate lysates or tissues. Positive control used Xenopus laevis and mouse kidney in WB and Xenopus laevis in IF. Western Blot Dilution: 1:1000 ELISA Dilution: 1:10,000 - 50,000 IF Microscopy Dilution: 1:5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.05 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: EFNB1/EFNB2/EFNB3

Andere Bezeichnung: EFNB1, Efnb2, EFNB3

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). Ephrin B1, B2, B3 are cell surface transmembrane ligands for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring. Ephrin B1 shows high affinity for the receptor tyrosine kinase EPHB1/ELK and can bind EPHB2 and EPHB3. Ephrin B3 may play a pivotal role in forebrain function. They binds to, and induces collapse of, commissural axons/growth cones in vitro. And may play a role in constraining the orientation of longitudinally projecting axons. Anti-Ephrin B1, B2, B3 Antibody is useful for researchers interested in Developmental Biology and Tyrosine Kinases/Adaptors.

Gen-ID: 1947

NCBI Accession: NP_004420

UniProt: P52800