CH25H Antikörper (C-Term)

Produktnummer ABIN7565948

Der Kaninchen Polyklonal Anti-CH25H-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, CH25H in Proben von Human zu detektieren.

Kurzübersicht für CH25H Antikörper (C-Term) (ABIN7565948)

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CH25H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-CH25H Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CH25H Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human CH25H.

Produktmerkmale: Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H

Aufreinigung: This affinity purified antibody is directed against human CH25H.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC.  Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates.  Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.92 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Andere Bezeichnung: CH25H

Hintergrund: Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.

Gen-ID: 9023

NCBI Accession: NP_003947

UniProt: O95992