MUC4 Antikörper

Produktnummer ABIN7565926

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MUC4 in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MUC4 Antikörper (ABIN7565926)

Target: MUC4 (Mucin 4, Cell Surface Associated (MUC4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MUC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-MUC4 Antikörper

Verwendungszweck: MUC4 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against human MUC4 protein.

Produktmerkmale: Synonyms: Rabbit Anti-MUC4 Antibody, Rabbit Anti-Mucin 4 Antibody, MUC4, Mucin-4, Ascites sialoglycoprotein, ASGP, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, Mucin-4 alpha chain, Ascites sialoglycoprotein 1, ASGP-1, Mucin-4 beta chain, Ascites sialoglycoprotein 2, ASGP-2, MUC

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human Mucin-4.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-MUC4 Antibody was tested in ELISA, Western Blot, and Immunohistochemistry. Antibodies shows no cross reactivity to non-mucilated proteins. Positive control used in WB were HPAC, HPAF-II, and Capan-2 cell lines and IHC was HPAC cell line. Immunohistochemistry Dilution: 1:1000 Western Blot Dilution: 1:1000 ELISA Dilution: 5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu MUC4

Target: MUC4 (Mucin 4, Cell Surface Associated (MUC4))

Andere Bezeichnung: MUC4

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). MUC4 (Mucin 4, Cell Surface Associated) is a Protein Coding gene. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. Highly glycosylated proteins called mucins, are the major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix. MUC4's ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. MUC4 seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. These glycoproteins play an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 Tyr-1248. In polarized epithelial cells segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a co-receptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. May play a role in tumor progression. MUC4 is associated with diseases such as bile duct cancer, keratitis, adenosquamous carcinoma, and pancreatic adenocarcinomas. Anti-MUC4 Antibody is useful for researchers interested in digestion, cancer research, and extracellular matrix Antibodies.

Gen-ID: 4585

NCBI Accession: NP_004523

UniProt: Q99102

Pathways: Regulation of Leukocyte Mediated Immunity