GAPDH Antikörper

Produktnummer ABIN7565915

Der Maus Monoklonal anti-GAPDH Antikörper wird verwendet zum Nachweis von GAPDH in Proben von Kaninchen. Er wurde validiert für WB, ELISA, IHC, IP, FM und MA.

Kurzübersicht für GAPDH Antikörper (ABIN7565915)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Klon: 33F2-C8-G6-E3

Produktdetails anti-GAPDH Antikörper

Verwendungszweck: GAPDH Antibody

Kreuzreaktivität (Details): Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase.

Produktmerkmale: Synonyms: mouse anti-GAPDH antibody, Loading Control Antibody, G3PDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

Aufreinigung: The antibody is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein.

Immunogen Type: Native Protein

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-GAPDH (Mouse) has been tested in ELISA and western blot. This product is suitable in IHC and IF. Specific conditions should be optimized by the end user. Expect a band of ~38 kDa in size corresponding to Glyceraldehyde 3-Phosphate Dehydrogenase protein by Western blot in the appropriate cell lysate or extract. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund: Background: GAPDH Loading Control Monoclonal Antibody detects Glyceraldehyde-3-Phosphate Dehydrogenase. GAPDH is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. Anti-GAPDH antibody is widely used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue and is ideal for Neuroscience, Cell Signaling and Cancer Research.

Gen-ID: 100009074

NCBI Accession: NP_001075722

UniProt: P46406