Streptavidin Antikörper (Biotin)

Produktnummer ABIN7565911

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Streptavidin in ELISA und DB. Er zeigt eine Reaktivität gegenüber Streptomyces avidinii.

Kurzübersicht für Streptavidin Antikörper (Biotin) (ABIN7565911)

Target: Streptavidin

Reaktivität: Streptomyces avidinii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Streptavidin Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Dot Blot (DB)

Produktdetails anti-Streptavidin Antikörper (Biotin)

Verwendungszweck: Streptavidin Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin.

Produktmerkmale: Synonyms: rabbit anti-Streptavidin Antibody biotin Conjugation, biotin conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin BAC Antibody

Aufreinigung: Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Streptavidin (Streptomyces avidinii)

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher. ELISA Dilution: 1:2,000-1:20,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Streptavidin

Target: Streptavidin

Hintergrund: Background: Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10-14 Mol/l to 10-15 Mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin through the antibody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interaction.