Glycerol Kinase Antikörper (HRP)

Produktnummer ABIN7565884

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Glycerol Kinase in ELISA und WB. Er zeigt eine Reaktivität gegenüber Cellulomonas.

Kurzübersicht für Glycerol Kinase Antikörper (HRP) (ABIN7565884)

Target: Glycerol Kinase (GK)

Reaktivität: Cellulomonas

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glycerol Kinase Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-Glycerol Kinase Antikörper (HRP)

Verwendungszweck: Glycerol Kinase Antibody HRP Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].

Produktmerkmale: Synonyms: goat anti-Glycerol Kinase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody

Aufreinigung: Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Glycerol Kinase [Cellulomonas species]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Glycerol Kinase has been tested by ELISA and western blot. this product is assayed against 1.0 μg of Glycerol Kinase [Cellulomonas species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Glycerol Kinase

Target: Glycerol Kinase (GK)

Andere Bezeichnung: glpK

Hintergrund: Background: Glycerol Kinase is a key enzyme in the regulation of glycerol uptake and metabolism. Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis.

Gen-ID: 6960048

UniProt: D0VZG4