GlnA Antikörper (HRP)

Produktnummer ABIN7565883

Produktdetails

Target: GlnA

Reaktivität: Microbial

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Verwendungszweck: Glutamine Synthetase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].

Produktmerkmale: Synonyms: goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase

Aufreinigung: Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Glutamine Synthetase [Microbial]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Glutamine Synthetase has been assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: GlnA

Hintergrund: Background: Glutamine Synthetase catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. Glutamine Synthetase is present predominantly in the brain, kidneys, and liver. In the brain it participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals. Glutamine Synthetase, in the brain, is found primarily is astrocytes.

Gen-ID: 3345165

UniProt: Q79VE3